S4045

Sigma

 

Monoclonal Anti-Splicing Factor SC-35 antibody produced in mouse

clone SC-35, ascites fluid

MDL number:MFCD00282472

Details

Related Products

References

Reviews

Description

Immunogenpartially purified mammalian splicesome.
SpecificityRecognizes a phospho-epitope on the non-snRNP (small nuclear ribonucleoprotein particles) factor SC-35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF. The antibody labels SC-35 as a speckled pattern in the nucleoplasm, excluding the nucleoli. Other uses include inhibition and depletion of splicing activity in nuclear extracts and immunoaffinity purification.

Properties

antibody formascites fluid
cloneSC-35 (monoclonal)
mol wtantigen mol wt 35 kDa (a doublet)
contains15 mM sodium azide
species reactivityrat, newt, frog, human, Drosophila
application(s)immunocytochemistry: suitable
 immunoprecipitation: suitable
 indirect ELISA: suitable
 indirect immunofluorescence: 1:2,000 using cultured human fibroblasts
isotypeIgG1
Gene Informationhuman ... SFRS2(6427)
 rat ... Sfrs2(494445)
shipped indry ice
storage temp.−20°C

Safety

WGK Germany3

References

referenceFu, X.-D., and Maniatis, T.I., Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature 343, 437-441, (1990) Abstract
 Spector, D.L., et al., Associations between distinct pre-mRNA splicing components and the cell nucleus. EMBO J. 10, 3467-3481, (1991) Abstract
 Fu, X.-D., et al., General splicing factors SF2 and SC35 have equivalent activities in vitro and both affect alternative 5' and 3' splice site selection. Proc. Natl. Acad. Sci. U. S. A. 89, 11224, (1992) Abstract
 Fu, X.-D., and Manatis, T.I. Science 256, 535-538, (1992) Abstract
 Carmo-Fonseca, M., et al., In vivo detection of snRNP-rich organelles in the nuclei of mammalian cells. EMBO J. 10, 1863-1873, (1991) Abstract
 Wang, J., et al., Localization of pre-messenger RNA at discrete nuclear sites. Proc. Natl. Acad. Sci. U. S. A. 88, 7391-7395, (1991) Abstract
 Wu, Z., et al., Small nuclear ribonucleoproteins and heterogeneous nuclear ribonucleoproteins in the amphibian germinal vesicle: loops, spheres, and snurposomes. J. Cell Biol. 113, 465-483, (1991) Abstract
 Carmo-Fonseca, M., et al., Transcription-dependent colocalization of the U1, U2, U4/U6, and U5 snRNPs in coiled bodies. J. Cell Biol. 117, 1-14, (1992) Abstract
 Jimenez-Garcia, L.F., and Spector, D.L., In vivo evidence that transcription and splicing are coordinated by a recruiting mechanism. Cell 73, 47-59, (1993) Abstract
 Gui, J.-F., et al., A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Nature 369, 678-682, (1994) Abstract
 Fu, X.-D., and Maniatis, T.I., The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site. Proc. Natl. Acad. Sci. U. S. A. 89, 1725-1729, (1992) Abstract
 Peterlin, B.M., et al., Interplay between 7SK snRNA and oppositely charged regions in HEXIM1 direct the inhibition of P-TEFb EMBO J. 24, 4291-4303, (2005) Abstract
 Melle, C., et al., Rad54B Targeting to DNA Double-Strand Break Repair Sites Requires Complex Formation with S100A11 Mol. Biol. Cell 19, 2926-2935, (2008) Abstract
 Jacquier, A., et al., Chronic activation in presymptomatic amyotrophic lateral sclerosis (ALS) mice of a feedback loop involving Fas, Daxx, and FasL Proc. Natl. Acad. Sci. U. S. A. 103, 6007-6012, (2006) Abstract